Downstream processing

Water in the broth filtrate can be removed by a simple evaporation process. The proteins can be extracted from the aqueous medium by forming reverse micelles.

The protein bound to the ligand can be eluted by reducing their interaction. This can be achieved by changing the pH of the buffer, altering the ionic strength or by using another free ligand molecule. The affinity interaction e. Downstream bioprocessing[ edit ] The downstream part of a bioprocess refers to the part where the cell mass from the upstream are processed to meet purity and quality requirements.

Addition of flocculating agents inorganic salt, organic polyelectrolyte, mineral hydrocolloid is often necessary to achieve appropriate flocculation. The ionic bound molecules can be eluted from the matrix by changing the pH of the eluant or by increasing the concentration of salt solution.

On the other hand, the larger molecules cannot pass through the pores and therefore come out first with the mobile liquid Fig.

The precipitation of proteins is dependent on several factors such as protein concentration, pH and temperature.


When they reach the desired density for batch and fed-batch cultures they are harvested and moved to the downstream section of the bioprocess.

The particles are trapped within the matrix and the fluid passes out. We ensure that our products comply with the latest environmental standards and that leaks are not an issue, regardless of how extreme the conditions. The upstream part of the production process does not do anything with the material itself, such as processing the material.

The cells and other solid particles get adsorbed on gas bubbles. The equipment is simple with low power consumption and is easy to operate. Formulation broadly refers to the maintenance of activity and stability of a biotechnological products during storage and distribution.

However, this procedure has a limitation since it cannot be used for large scale separation of volatile products due to cost factor. The cells break as they are forced against the wall of the vessel by the beads.

Such proteins can be eluted by employing solutions in chromatography. Tubular bowl centrifuge Fig. The body of the cell disrupter is kept cool while the operation is on. Extraction of lipophilic products can be done by the following techniques. But this technique can be used only for a very few heat-stable intracellular products.

The cells are disrupted by the forces created at the point of contact. The distribution of the desired product is based on its surface and ionic character and the nature of phases.

A company that combines both upstream and downstream processes is an integrated company. Several methods are in use for solid-liquid separation. Regardless of the industry involved, the downstream process has direct contact with customers through the finished product.

Precipitation is the most commonly used technique in industry for the concentration of macromolecules such as proteins and polysaccharides.


Among the physical methods of cell disruption described above, ultra sonication, high-pressure homogenization, impingement and grinding with glass beads are mechanical while osmotic shock and heat shock are non-mechanical.

Depending on the product and its intended use, polishing may also include operations to sterilize the product and remove or deactivate trace contaminants which might compromise product safety.

Downstream Processing

The sponge-like gel beads with pores serve as molecular sieves for separation of smaller and bigger molecules. Membrane filtration has become a common separation technique in industrial biotechnology.

The downstream stage further includes the actual sale of that product to other businesses, governments or private individuals. The particles are trapped within the matrix and the fluid passes out. It involves the separation of molecules based on their surface charges.

Cell disruption by enzymatic methods has certain advantages i.

Downstream processing

There is a continuous feeding of the slurry and collection of clarified fluid, while the solids deposited can be removed intermittently.

Ultrasonic disintegration is widely employed in the laboratory.

The Definitions of

Jun 29,  · The production process like a river. Upstream refers to the material inputs needed for production, while downstream is the opposite end, where products get produced and distributed. Nov 09,  · Recent developments in downstream processing of biomolecules — including continuous processing, bind–elute affinity capture, and flow-through polishing steps — have increased the need for greater selectivity from chromatography adsorbents.

The downstream part of a bioprocess refers to the part where the cell mass from the upstream are processed to meet purity and quality requirements.

Downstream processing is usually divided into three main sections: cell disruption, a purification section and a polishing section. Downstream processing is an area ripe for technology innovations. The need to meet high upstream titers and the demand for lower cost are pushing companies to look at more creative strategies in purification and recovery.

2 1 Downstream Processing of Biotechnology Products macologically active compounds in cosmetics. Currently only a few proteins are used in this area. The most prominent one is the botulinum toxin, Botox ®, used for skin care [4].

ADVERTISEMENTS: This article throws light upon the five stages in downstream processing. The five stages are: (1) Solid-Liquid Separation (2) Release of Intracellular Products (3) Concentration (4) Purification by Chromatography and (5) Formulation.

In outline of the major steps in downstream processing is given. Stage # 1.

The Definitions of Downstream processing
Rated 4/5 based on 9 review
Bioprocess - Wikipedia